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Structural, dimensional, and functional characteristics of isolated liver mitochondria.
These data are excerpted from:
Adrian R.L.Gear and Jana M.Bednarek, “Direct counting and sizing of Mitochondria in solution”,
The Journal of Cell Biology (1972), 54, pp. 325–345.
1.Characterization of light and heavy liver mitochondrial fractions.
Heavy mitochondria. | Parameter | Specific content per mg protein | Particle content
(units per particle x 10-9 ) | Number of particles | 3.95 x 109
| ----- | Diameter by Coultier counter | 1.02 micron | ----- | Diameter by electron
microscopy | 0.99 micron | ------- | Volume | 0.55 micron3 | ------- | DNA | 0.155
microg | 0.0392 microg | RNA | 9.0 microg | 2.28 microg | Cytochrome
a+a3 | 0.49 nmol | 0.124 nmol | Cyt. c oxidase activity,
k-1 | 45 | 11.4 | Total iron, nmol | 4.1 | 1.04 |
Light mitochondria:
| Parameter |
Specific content per mg protein |
Paritcle content
(units per particle x 10-9 ) |
Number of particles |
5.14 x 109 |
---- |
Diameter by Coultier counter |
0.82 micron |
--- |
Diameter by electron microscopy |
0.72 micron |
---- |
Volume |
0.3 micron3 |
--- |
DNA |
0.245 microg |
0.0477 microg |
RNA |
13.8 microg |
2.68 microg |
Cytochrome a+a3 |
0.66 nmol |
0.128 nmol |
Cyt. c oxidase activity, k-1 |
70 |
13.6 |
Total iron, nmol |
5.3 |
1.03 |
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Last Updated ( Saturday, 12 May 2007 )
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